Intracytoplasmic sperm injection

 

Sperm is released via a pipette into the cytoplasm of a secondary oocyte which is held in position by gentle suction through glass holding pipette. Sperm is then injected into the cytoplasm. It is used to treat oligospermia.

With the extensive experiments of Iritani and Hosoi (1988) and other investigators, animal microfertilisation by injection is an established procedure and now referred to as intracytoplasmic sperm injection (ICSI).  Injection of sperm for microfertilisation can be done into the perivitelline space, cytoplasm and other areas. The combination of sperm and egg are left in appropriate incubation media for fertilization and subsequent growth. It was first performed in the human for clinical treatment of oligospermia by Trounson in 1990 in Melbourne, Australia.

 

 

    

 

 

 

 

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