High technology Medicine

Recombinant DNA

        Introduction to gene technology

        Gene injection
   Fluorescent in situ hybridization

        Southern blot

        Northern blot

        Polymerase chain reaction

        DNA sequencing

        Restricted fragment length polymorphism (RFLP)

        Single strand conformational polymorphism (SSCP)

        Denaturing gradient gel electrophoresis (DGGE)

        Protein truncation test (PTT)

        Reverse transcriptase PCR

        RNAse cleavage

        Oligonucleotide specific hybridization (OSH)

        Microarrays
Ethics of gene technologies


Reproductive technologies

Techniques involving sperm

        Artificial insemination

            By donor (AID or donor insemination)

            By husband (AIH)

        Sperm intrafallopian transfer

        Intra-uterine insemination (IUI)

        Direct peritoneal insemination

        Microsurgical sperm retrieval

Techniques involving eggs

        Tubal ovum transfer

        Superovulation

        Egg recovery

        In vitro maturation

        In vivo oocyte maturation

Techniques involving both sperm and eggs

        Gamete intrafallopian transfer (GIFT)

        Peritoneal oocyte and sperm transfer (POST)

        Intracytoplasmic sperm injection (ICSI)

        Natural cycle oocyte retrieval intra-vaginal fertilization (NORIF)

        Pronuclear stage tubal transfer (PROST)

Techniques involving external fertilization of gametes

        In vitro fertilization

        In vitro maturation and fertilization
    Preimplantation diagnosis

        Removal of cleavage blocks

        Gamete and oviductal coculture

Techniques of embryo transfer

        Embryo transcervical transfer

        Embryo oviductal transfer or ZIFT

        Surrogate embryo transfer

        Assisted hatching

        Xenogenous egg incubation

Techniques of preserving gametes and early embryos

        Preservation of gametes

        Preservation of embryos


In vivo technologies

        Sperm transfer

        Sperm capacitation

        Sperm transport

        Egg transport

        Fertilization

        Oocyte maturation

        Implantation

      Ethics of in vitro fertilization
Ethics of cloning


In Cloning
Somatic cell nuclear transfer
(adult cell cloning)

Embryo cloning



Baby sex selection
Microsort technique
Kit with ovulation methods
Cervical mucus



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