Southern blot

The technique now known as Southern blot was first described by a man known as E.M. Southern in 1975 in Edinburgh. It is useful method of identifying gene or gene sequence in any tissue of the body of an individual without necessarily obtaining the cell in which the gene is active). As mentioned earlier, this is based on the  principle that all genes are present in all cells of the body repressed, but only active or are derepressed in the cell or tissue of expression. For diagnosis, we need a gene probe, i.e. a sequence of nucleotides identifical to the one being sought after for diagnosis but which does not contain repetitive DNAs that might confuse the blotting process. Sites containing repititive genes are available in genome and may have repeats up to 30000 times. Hence a gene probe to be used in molecular blot should not contain repetitive DNA or interpretation of hybridisation will be difficult. This gene probe must also contain only exons and not introns. Since cDNA normally has the above properties, it is highly favoured in Southern blot techniques It is normally labelled with radioactive material. A good example of such radioactive materials could be 32P.

 

 

 

 

 

 

 

 

 

 

 

 

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