Sanger's method of gene sequencing

Sanger designed his method while working in Cambridge. In his method dideoxynucleotides are incorporated into DNA molecules to be sequenced. They differ from the deoxynucleotides in that they do not have the hydroxyl group in position 3. In view of the absence of OH at position 3, wherever there is incorporation of dideoxynucleotide there will be a break in the nucleotide chain since it is the position 3 hydroxyl group that makes the linkage with other nucleotide bases. Hence several fragments of the DNA are obtained by this method. These fragments are then subjected to electrophoresis using an electric current source.








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