Nick translation

This is a method for the labeling of DNA for use in molecular blots. It utilizes DNase I to create single stranded nicks in double stranded DNA. It then uses 5’3’ E coli exonulease to break the DNA at the nicks, followed by E coli 5’3’polymerase to incorporate new strand made by incorporation of labeled deoxyribonucleotides thereby producing the nick at the ends of the new strands moving along the 5’ to 3’ direction. Denaturing the double stranded DNA produces short part labeled single stranded DNA which can be used as gene probes.

 

 

 

 

 

 

 

 

 

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