Sperm capacitation


IIt is already known that in vivo manipulation of the sperm for capacitation is carried out in the female reproductive tract. The implication of the oviduct in the process is presently well recognized. Modern methods are being developed which can allow capacitation of sperm of any animal species in co-culture with relevant parts or zones of the oviduct as explants. The technique is so successful that it can even be used for xenogenous capacitation. Presently, molecular studies are being carried out extensively on the phenomena of sperm maturation and capacitation which must occur before the sperm cells are able to fertilize the egg.

Capacitation media have been developed to meet the in vitro requirements for ART in animals. Some investigators may use uterine fluid. Most animal sperms (excepting farm animals) can now be capacitated in Tyrode’s or Krebs-Ringer bicarbonate solution only. For the ram, bull and boar’s sperm, the technique of  is generally followed. Spermatozoa are allowed to stand at  20o C for 4 hours after washing. This is the stood-washed procedure.

Capacitation of boar sperm follows the modern method in which ejaculated boar spermatozoa are filtered through gauze in order to remove seminal plasma clots. They are then centrifuged at 200g for 3 minutes in 0.9% saline which contains 1mg-ml of bovine serum albumin, at pH 7.2. The spermatozoa are then further washed by centrifugation at 1200g three times at three minutes each.




Capacitation of sperm








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